季泉江

时间:2018-09-04浏览:11517设置

季泉江课题组先容


Principal Investigator
Quanjiang Ji (季泉江),Assistant Professor
Address: 393 Huaxia Middle Road, Pudong, Shanghai, China, 200120

Email: quanjiangji@shanghaitech.edu.cn


Assistant Professor, ShanghaiTech University, 2016-current;
Postdoc., University of California, Berkeley (Advisor: Prof. Michelle Chang), 2014-2016;
Ph.D., University of Chicago (Advisor: Prof. Chuan He), 2009-2014;
B.S., Nanjing University, 2005-2009.


Awards:

NSFC for Excellent Young Scholars (国家优青),2019;
Shanghai Science and Technology Committee Rising-Star Program (上海市青年科技启明星),2019;
Young Overseas High-Level Talents Introduction Plan, 2016;
Camille and Henry Dreyfus Postdoctoral Fellowship, 2015;
Chinese Government Award for Outstanding Self-Financed Students Abroad, 2013;
The Everett E Gilbert Memorial Prize, 2012.


Research

The emergence of drug-resistant human pathogens has posed a severe public crisis worldwide. To counter infections caused by major human pathogens, we aim to create novel genome editing tools, address fundamental infection and drug-resistant mechanisms, and develop new therapeutic means with the utilization of multiple approaches (chemical, biological, and engineering).

Genome editing in human pathogens: Genetics is the key means to study bacterial physiology. However, traditional genetic manipulation methods in major human pathogens remain as time-consuming and laborious endeavors. We have created rapid and highly efficient genetic manipulation tools in multiple major human pathogens, including Staphylococcus aureus (JACS, 2017; Chem Sci, 2018), Pseudomonas aeruginosa (iScience, 2018), Klebsiella pneumoniae (Appl Environ Microbiol, 2018; Antimicrob Agents Chemother, 2019), and Acinetobacter baumannii (Cell Chem Biol, 2019) by engineering the powerful CRISPR/Cas9 genome editing technology and deaminase-mediated base editing systems. These tools have been requested and utilized by numerous research groups worldwide and are available in Addgene (http://www.addgene.org/Quanjiang_Ji/).

We utilize protein engineering and synthetic biology approaches to develop genome-wide screening tools and aim to construct label-free gene-knockout libraries in multiple human pathogens. The development of these tools will advance fundamental physiology studies as well as novel drug-target exploration.

We study fundamental DNA recognition and cleavage mechanisms of CRISPR systems (PLOS Biol, 2019). We design, engineer, and functionalize CRISPR systems for diverse applications, in particular in the study of infectious diseases.


Fundamental infection and drug-resistant mechanisms: We study basic infection and drug-resistant mechanisms in major human pathogens by taking advantages of structural biology and genome editing approaches (PNAS, 2018; Mol Microbiol, 2018). Recently, we focused on elucidating the molecular mechanisms of nicotianamine-like metallophore-mediated transition metal acquisition processes as well as biofilm-formation mechanisms of several cell surface proteins in human pathogens. We are also interested in small-molecule signaling and regulatory pathways that affect bacterial pathogenesis and drug resistance.

Therapeutic means against infections: We develop therapeutic antibodies and small molecules targeting key virulence or drug-resistant proteins, in particular the extracellular proteins that play vital roles in bacterial metal acquisition and biofilm formation. We aim to identify new drug targets using the mutant libraries we are currently constructing and screen effective antibodies or small molecules against them.



Publications

Book Chapters


1. Chen, W. & Ji, Q.* (2020) Genetic manipulation of MRSA using CRISPR/Cas9 technology. Methods Mol Biol 2069:113-124.


Research Articles and Reviews


2019


11. Chen, W., Zhang, H., Zhang, Y., Wang, Y., Gan, J.*, Ji, Q.* (2019) Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease. PLoS Biol 17: e3000496.


10. Wang, Y., Wang, Z., Chen, Y., Hua, X., Yu, Y., Ji, Q.* (2019) A highly efficient CRISPR-Cas9-based genome engineering platform in Acinetobacter baumannii toward the understanding of H2O2-sensing mechanism of OxyR. Cell Chem Biol doi: 10.1016/j.chembiol.2019.09.003.


9. Fu, T., Liu, L., Yang, Q.L., Wang, Y., Xu, P., Zhang, L., Liu, S., Dai, Q., Ji, Q., Xu, G.L., He, C., Luo, C., Zhang, L. (2019) Thymine DNA glycosylase recognizes the geometry alteration of minor grooves induced by 5-formylcytosine and 5-carboxylcytosine. Chem Sci 10: 7407-17.


8. He, T., Wang, R., Liu, D., Walsh, T.R., Zhang, R., Lv, Y., Ke, Y., Ji, Q., Wei, R., Liu, Z., Shen, Y., Wang, G., Sun, L., Lei, L., Lv, Z., Li, Y., Pang, M., Wang, L., Sun, Q., Fu, Y., Song, H., Hao, Y., Shen, Z., Wang, S., Chen, G., Wu, C., Shen, J., Wang, Y. (2019) Emergence of plasmid-mediated high-level tigecycline resistance genes in animals and humans. Nat Microbiol 4: 1450-6.


7. Sun, Q. #, Wang, Y. #, Dong, N., Shen, L., Zhou, H., Hu, Y., Gu, D., Chen, S., Zhang, R.*, Ji, Q.* (2019) Application of CRISPR/Cas9-based genome editing in studying the mechanism of pandrug resistance in Klebsiella pneumoniae. Antimicrob Agents Chemother. 63: e00113-19.


2018


6. Wang, Y., Wang, S., Chen, W., Song, L., Shen, Z., Yu, F., Li, M., Ji, Q.*(2018) Precise and efficient genome editing in Klebsiella pneumoniaeusing CRISPR-Cas9 and CRISPR-assisted cytidine deaminase. Appl Environ Microbiol 84: e01834-18.


5. Chen, W., Zhang, Y., Zhang, Y., Pi, Y., Gu, T., Song, L., Wang, Y., Ji, Q.* (2018) CRISPR/Cas9-based genome editing in Pseudomonas aeruginosa and cytidine deaminase-mediated base editing in Pseudomonas species. iScience 6: 222-31.


4. Wei, W.#, Zhang, Y.#, Gao, R., Li, J., Xu, Y., Wang, S., Ji, Q.*, Feng, Y.* (2018) Crystal structure and acetylation of BioQ suggests a novel regulatory switch for biotin biosynthesis in Mycobacterium smegmatis. Mol Microbiol 109: 642-62.


3. Song, L., Zhang, Y., Chen, W., Gu, T., Zhang, S.Y., Ji, Q.* (2018) Mechanistic insights into staphylopine-mediated metal acquisition.  Proc Natl Acad Sci U S A 115: 3942-7.


2. Gu, T.#, Zhao, S.#, Pi, Y., Chen, W., Chen, C., Liu, Q., Li, M., Han, D.*, Ji, Q.* (2018) Highly efficient base editing in Staphylococcus aureus using an engineered CRISPR RNA-guided cytidine deaminase. Chem Sci9: 3248-53.


2017


1. Chen, W., Zhang, Y., Yeo, W.S., Bae, T., Ji, Q.* (2017) Rapid and efficient genome editing in Staphylococcus aureus by using an engineered CRISPR/Cas9 system. J Am Chem Soc 139: 3790-5.



(# co-first author; * corresponding author)



Patents

8. 季泉江、王宇。一种用于鲍曼不动杆菌胞嘧啶碱基编辑质粒及其应用。申请号:201910644444.1
7. 季泉江、王宇。双质粒系统及其应用。申请号:201910644324.1
6. 季泉江、王宇。一种用于肺炎克雷伯菌基因编辑的双质粒系统。申请号:201811039504.9
5. 季泉江、王宇。一种肺炎克雷伯菌基因编辑的表达载体。申请号:201811039489.8
4. 季泉江、陈未中。一种pnCasPA-BEC质粒及其应用。申请号:201810767194.6
3. 季泉江、陈未中。一种pCasPA/pACRISPR双质粒系统及其应用。申请号:201810766759.9
2. 季泉江、顾桐年。一种pnCasSA-BEC质粒及其应用。申请号:201810169946.9
1. 季泉江、陈未中。一种pCasSA质粒及其应用。申请号:201611255504.3



Group Activities



Current Group Members

Weizhong Chen(陈未中)

Research assistant professor
Research assistant professor, ShanghaiTech University, 2018-Current;
Postdoc., ShanghaiTech University, 2016-2018;
Ph.D., University of Science and Technology of China, 2009-2015;
B.S., University of Science and Technology of China, 2005-2009.

Email: chenwzh@shanghaitech.edu.cn

Zhaowei Wu (吴兆韡)

Postdoc
Postdoc., ShanghaiTech University, 2018-Current;
Ph.D., Northwest A&F University, 2014-2018;
B.S., Northwest A&F University, 2010-2014.

Email: wuzw1@shanghaitech.edu.cn

Tongnian Gu (顾桐年)

Graduate student
Graduate student, ShanghaiTech University, 2015-Current;
B.S., University of Electronic Science and Technology of China 2008-2012.

Email: gutn@shanghaitech.edu.cn

Yifei Zhang (张翼飞)

Graduate student
Graduate student, ShanghaiTech University, 2016-Current;
B.S., Zhengzhou University, 2012-2016.

Email: zhangyf1@shanghaitech.edu.cn

Yishuang Pi (皮义双)

Graduate student
Graduate student, ShanghaiTech University, 2017-Current;
B.S., Shenyang Pharmaceutical University, 2013-2017.

Email: piysh@shanghaitech.edu.cn

Ya Zhang (张雅)

Graduate student
Graduate student, ShanghaiTech University, 2017-Current;
B.S., Southwest University, 2013-2017.

Email: zhangya@shanghaitech.edu.cn

Zhipeng Wang(王志鹏)

Graduate student
Graduate student, ShanghaiTech University, 2018-Current;
B.S., Zhengzhou University, 2014-2018.

Email: wangzhp@shanghaitech.edu.cn

Yujue Wang(王玉珏)

Graduate student
Graduate student, ShanghaiTech University, 2018-Current;
B.S., Shandong University, 2014-2018.

Email: wangyj6@shanghaitech.edu.cn

Hongyuan Zhang (张洪源)

Graduate student
Graduate student, ShanghaiTech University, 2018-Current;
B.S., Shandong University, 2014-2018.

Email: zhanghy2@shanghaitech.edu.cn

Na Tang(汤娜)

Graduate student
Graduate student, ShanghaiTech University, 2019-Current;
B.S., China Pharmaceutical University, 2015-2019.

Email: tangna@shanghaitech.edu.cn

Minghui Lv(吕明慧)

Graduate student
Graduate student, ShanghaiTech University, 2019-Current;
B.S., Shandong Normal University, 2015-2019.

Email: lvmh@shanghaitech.edu.cn

Yannan Wang(王艳男)

Graduate student
Graduate student, ShanghaiTech University, 2019-Current;
B.S., Wuhan University of Technology, 2015-2019.

Email: wangyn5@shanghaitech.edu.cn

Chang Liu (刘畅)

Undergraduate student
Undergraduate student, ShanghaiTech University, 2017-Current;

Email: liuchang2@shanghaitech.edu.cn


Alumni

Liqiang Song (宋立强)

Postdoc   2016-2018
Current: UT Health Science Center (Postdoc)

Yu Wang (王宇)

Postdoc   2017-2019
Current: Jiangxi Agricultural University (Associate Professor) 

Yani Zhao (赵亚妮)

Undergraduate   2017-2019
Current: UNC at Chapel Hill (Ph.D. candidate)


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